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Nucleotide sequencing of the SalI j region of the virulent Malawi (LIL20/1) strain of African swine fever virus (ASFV) identified an open reading frame (ORF), designated j9L, with extensive similarity to the family of protein kinases. This ORF encodes a 35.1-kDa protein of 299 amino acids which shares 24.6% amino acid identity with the human pim-1 proto-oncogene and 21.0% identity with the vaccinia virus B1R-encoded protein kinase. The ASFV ORF contains the motifs characteristic of serine-threonine protein kinases, with the exception of the presumed ATP-binding site, which is poorly conserved. The ORF was expressed to high levels in Escherichia coli, and the recombinant enzyme phosphorylated a calf thymus histone protein on serine residues in vitro. An antibody raised to an amino-terminal peptide of the ASFV protein kinase was reactive with the recombinant protein in Western immunoblot analyses and was used to demonstrate the presence of the protein kinase in ASF virions.

Type

Journal article

Journal

J virol

Publication Date

08/1993

Volume

67

Pages

4549 - 4556

Keywords

African Swine Fever Virus, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genes, Viral, Humans, Malawi, Molecular Sequence Data, Open Reading Frames, Plasmids, Protein Kinases, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-pim-1, Proto-Oncogenes, Recombinant Proteins, Restriction Mapping, Sequence Homology, Amino Acid, Substrate Specificity, Vaccinia virus, Virion