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Innate and adaptive cellular immunity is dependent on interactions of cell surface receptors that initiate signaling, resulting in the formation of the immunological synapse and targeted delivery of effector functions. There has been considerable interest over the past 30 years in methods for isolating the extracellular regions of these receptors and components of the cytoplasmic signaling networks. This chapter describes our current protein expression toolkit used for structural studies of signaling proteins and the functional reconstitution of model cell surfaces, which comprises both bacterial and mammalian cell-based protein expression methodologies.

Original publication

DOI

10.1007/978-1-4939-6881-7_28

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2017

Volume

1584

Pages

451 - 472

Keywords

Adhesion, Affinity, Chromatography, Glycosylation, Inclusion bodies, Protein folding, Receptors, Animals, Gene Expression, Humans, Recombinant Proteins, Signal Transduction