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Upper-body adiposity is associated with increased metabolic disease risk, while lower-body adiposity is paradoxically protective. Efforts to understand the underlying mechanisms require appropriate and reproducible in vitro culture models. We have therefore generated immortalised (im) human preadipocyte (PAD) cell lines derived from paired subcutaneous abdominal and gluteal adipose tissue. These cell lines, denoted imAPAD and imGPAD display enhanced proliferation and robust adipogenic capacities. Differentiated imAPAD and imGPAD adipocytes synthesize triglycerides de novo and respond lipolytically to catecholamine-stimulation. Importantly the cells retain their depot-of-origin 'memory' as reflected by inherent differences in fatty acid metabolism and expression of depot-specific developmental genes. These features make these cell lines an invaluable tool for the in vitro investigation of depot-specific human adipocyte biology.

Original publication

DOI

10.1080/21623945.2016.1277052

Type

Journal article

Journal

Adipocyte

Publication Date

02/01/2017

Volume

6

Pages

40 - 55

Keywords

abdominal, adipogenesis, body fat distribution, gluteal, human adipose tissue, preadipocyte cell lines, Abdominal Fat, Adipocytes, Adipogenesis, Adipose Tissue, Adiposity, Body Fat Distribution, Buttocks, Cell Differentiation, Cell Line, Humans, Models, Biological, Obesity, Subcutaneous Fat