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Disruption of epigenetic regulators of transcription is a central mechanism of oncogenesis. Many of the advances in the understanding of these mechanisms are attributable to the successful development of chromatin immunoprecipitation (ChIP) for in vivo detection of histone modifications as well as chromatin binding regulatory proteins. This is a powerful technique for analyzing histone modifications as well as binding sites for proteins that bind either directly or indirectly to DNA. Here we present two ChIP protocols. The first is particularly useful for identifying histone modifications or binding at specific, known genomic sites. The second, employing serial analysis of gene expression, is particularly powerful for the discovery of previously unidentified sites of modification or binding.

Original publication

DOI

10.1007/978-1-59745-418-6_21

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2009

Volume

538

Pages

409 - 423

Keywords

Chromatin, Chromatin Immunoprecipitation, Cross-Linking Reagents, DNA Primers, DNA-Binding Proteins, Epigenesis, Genetic, Histones, Humans, Polymerase Chain Reaction, Sequence Analysis, DNA