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The major positive regulatory activity of the human alpha-globin gene complex has been localized to an element associated with a strong erythroid-specific DNase I hypersensitive site (HS -40) located 40 kb upstream of the zeta 2-globin mRNA cap site. Footprint and gel shift analyses of the element have demonstrated the presence of four binding sites for the nuclear factor GATA-1 and two sites corresponding to the AP-1 consensus binding sequence. This region resembles one of the major elements of the beta-globin locus control region in its constitution and characteristics; this together with evidence from expression studies suggests that HS -40 is a primary element controlling alpha-globin gene expression.

Type

Journal article

Journal

Mol Cell Biol

Publication Date

1991

Volume

11

Pages

4679 - 4689

Keywords

Base Sequence Dna Deoxyribonuclease I/metabolism Drug Resistance/genetics Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Globins/*genetics Human Molecular Sequence Data *Multigene Family Neomycin/pharmacology *Regulatory Sequences, Nucleic Acid Restriction Mapping Transformation, Genetic