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The purpose of this study was to test the hypothesis that energy metabolism is impaired in residual intact myocardium of chronically infarcted rat heart, contributing to contractile dysfunction. Myocardial infarction (MI) was induced in rats by coronary artery ligation. Hearts were isolated 8 wk later and buffer-perfused isovolumically. MI hearts showed reduced left ventricular developed pressure, but oxygen consumption was unchanged. High-energy phosphate contents were measured chemically and by 31P-NMR spectroscopy. In residual intact left ventricular tissue, ATP was unchanged after MI, while creatine phosphate was reduced by 31%. Total creatine kinase (CK) activity was reduced by 17%, the fetal CK isoenzymes BB and MB increased, while the "adult" mitochondrial CK isoenzyme activity decreased by 44%. Total creatine content decreased by 35%. Phosphoryl exchange between ATP and creatine phosphate, measured by 31P-NMR magnetization transfer, fell by 50% in MI hearts. Thus, energy reserve is substantially impaired in residual intact myocardium of chronically infarcted rats. Because phosphoryl exchange was still five times higher than ATP synthesis rates calculated from oxygen consumption, phosphoryl transfer via CK may not limit baseline contractile performance 2 mo after MI. In contrast, when MI hearts were subjected to acute stress (hypoxia), mechanical recovery during reoxygenation was impaired, suggesting that reduced energy reserve contributes to increased susceptibility of MI hearts to acute metabolic stress.

Original publication

DOI

10.1172/JCI117756

Type

Journal article

Journal

J Clin Invest

Publication Date

03/1995

Volume

95

Pages

1092 - 1100

Keywords

Adenosine Diphosphate, Adenosine Triphosphate, Animals, Citrate (si)-Synthase, Creatine Kinase, Energy Metabolism, Glycolysis, Hypoxia, In Vitro Techniques, Isoenzymes, Magnetic Resonance Spectroscopy, Myocardial Contraction, Myocardial Infarction, Oxygen Consumption, Perfusion, Phosphates, Phosphocreatine, Rats, Stress, Physiological, Ventricular Pressure