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Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro through blocking of protein:protein interaction with the co-receptor CCR5. The bead display approach has many potential applications for probing biological systems and for drug lead development.

Original publication

DOI

10.1038/srep03030

Type

Journal article

Journal

Sci Rep

Publication Date

23/10/2013

Volume

3

Keywords

Anti-HIV Agents, HIV Envelope Protein gp120, HIV-1, High-Throughput Screening Assays, Humans, Kinetics, Microbial Sensitivity Tests, Microspheres, Peptide Library, Peptides, Protein Binding, Receptors, CCR5, Receptors, HIV, Reproducibility of Results, beta 2-Microglobulin