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Few target epitopes have been described for human CD8 T lymphocytes in antigens of Mycobacterium tuberculosis. By use of a reverse immunogenetics approach, 23 motif-bearing peptides of the Ag85 complex were tested for binding to HLA-B*35, one of the common B-types in West Africa. Three 9-mer peptides bound with high affinity to HLA-B*3501 and displayed low dissociation rates of peptide-major histocompatibility complexes (MHCs). IC(50) and half-life values of peptide-MHC class I complexes were in the same range as reported earlier for other immunogenic peptides. Immune responses against peptide Ag85C (aa 204-212) WPTLIGLAM were characterized in detail. Peptide-stimulated effector cells were able to kill macrophages infected with M. tuberculosis or bacille Calmette-Guérin. Peptide-specific CD8 T cells could be visualized by using HLA-B*3501 tetramers and were shown to produce interferon-gamma and tumor necrosis factor-alpha. Together with other published epitopes, these peptides can be used to study more closely the role of CD8 T cells in mycobacterial infection and tuberculosis.

Original publication

DOI

10.1086/319267

Type

Journal article

Journal

J Infect Dis

Publication Date

15/03/2001

Volume

183

Pages

928 - 934

Keywords

Acyltransferases, Algorithms, Amino Acid Motifs, Antigens, Bacterial, CD8-Positive T-Lymphocytes, Cells, Cultured, Cytotoxicity Tests, Immunologic, Epitopes, T-Lymphocyte, HLA-B35 Antigen, Humans, Interferons, Macrophages, Mycobacterium tuberculosis, Peptides