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CD30 positive anaplastic large cell lymphoma (ALCL) is a type of non-Hodgkin's lymphoma associated with a specific chromosome translocation between chromosomes 2 and 5. Recent molecular characterization of the translocation breakpoint has identified a gene fusion between NPM (nucleophosmin) and ALK (anaplastic lymphoma kinase). Using a DNA hybridization technique, the NPM rearrangement was found among 5/5 ALCL samples. We have developed a PCT methodology which has enabled the detection of the NPM-ALK rearrangements amongst seven t(2;5)(p23;q35) ALCL cases based on a long-range PCR of genomic DNA. The rapidity and robustness of this method may have diagnostic applications for ALCL.

Type

Journal article

Journal

Br J Haematol

Publication Date

04/1995

Volume

89

Pages

905 - 907

Keywords

Base Sequence, Blotting, Southern, Gene Rearrangement, Humans, Lymphoma, Large-Cell, Anaplastic, Molecular Sequence Data, Nuclear Proteins, Nucleic Acid Hybridization, Polymerase Chain Reaction, Protein-Tyrosine Kinases, Receptor Protein-Tyrosine Kinases