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We have recently reported the development of a multiplex, real-time quantitative polymerase chain reaction (PCR) assay for RhD zygosity determination based on the coamplification and quantification of RHD-specific sequences in relation to a reference gene, albumin. This paper discusses the optimization procedure and technical parameters of this multiplex assay.

Original publication

DOI

10.1111/j.1749-6632.2001.tb03879.x

Type

Conference paper

Publication Date

09/2001

Volume

945

Pages

156 - 160

Keywords

Albumins, Heterozygote, Homozygote, Humans, Polymerase Chain Reaction, Reproducibility of Results, Rh-Hr Blood-Group System