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The routes used by antigen-presenting cells (APC) to convert the transmembrane fusion glycoprotein (F) of measles virus (MV) to HLA class I and class II presentable peptides have been examined, using cloned cytotoxic T lymphocytes in functional assays. Presentation by Epstein-Barr virus-transformed B lymphoblastoid cell lines was achieved using live virus, ultraviolet light-inactivated virus, and purified MV-F delivered either as such or incorporated in immunostimulating complexes (MV-F-ISCOM). Only live virus and MV-F-ISCOM allow presentation by class I molecules, while all antigen preparations permit class II-restricted presentation. We observe presentation of MV-F from live virus and as MV-F-ISCOM by class II molecules in a fashion that is not perturbed by chloroquine. Our studies visualize novel presentation pathways of type I transmembrane proteins.

Type

Journal article

Journal

J Exp Med

Publication Date

01/07/1992

Volume

176

Pages

119 - 128

Keywords

Animals, Antigen-Presenting Cells, Antigens, CD4, Antigens, CD8, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Humans, Lymphocyte Activation, Measles virus, T-Lymphocytes, Cytotoxic, Viral Fusion Proteins