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Current models suggest that tissue-specific genes are arranged in discrete, independently controlled segments of chromatin referred to as regulatory domains. Transition from a closed to open chromatin structure may be an important step in the regulation of gene expression. To determine whether the human alpha-globin cluster, like the beta-globin cluster, lies within a discrete, erythroid-specific domain, we have examined the long-range genomic organization and chromatin structure around this region. The alpha genes lie adjacent to at least four widely expressed genes. The major alpha-globin regulatory element lies 40 kb away from the cluster within an intron of one of these genes. Therefore, unlike the beta cluster, cis-acting sequences controlling alpha gene expression are dispersed within a region of chromatin that is open in both erythroid and nonerythroid cells. This implies a difference in the hierarchical control of alpha- and beta-globin expression.

Type

Journal article

Journal

Cell

Publication Date

29/05/1992

Volume

69

Pages

781 - 793

Keywords

Animals, Blotting, Northern, Cell Line, Chromatin, Deoxyribonuclease I, Gene Expression Regulation, Globins, Humans, Introns, Methylation, Multigene Family, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Tumor Cells, Cultured