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Dendritic cell (DC)-based immunization represents a promising approach for the immunotherapy of cancer. The optimal conditions required to prepare DCs remain to be defined. Monocytes incubated in the presence of interferon (IFN)-beta and interleukin (IL)-3 give rise to a distinct type of DCs (IFN-beta/IL-3 DCs) that are particularly efficient at eliciting IFN-gamma and IL-5 production by allogeneic helper T cells. We assessed the capacity of this new type of DCs to prime antigen-specific naive CD8(+) T cells and compared them to the conventional DCs differentiated in the presence of granulocyte-macrophage colony stimulating factor (GM-CSF) and IL-4 (GM-CSF/IL-4 DCs). We demonstrate that IFN-beta/IL-3 DCs matured by TLR3 or CD40 ligation efficiently prime Melan-A(26-35)-specific CD8(+) T cells in vitro, at a similar level as GM-CSF/IL-4 DCs. Activated antigen-specific CD8(+) T cells produced IFN-gamma and displayed potent cytotoxic activity against peptide-pulsed target cells. Expansion of CD8(+) T cell numbers was generally higher following priming with CD40-L than with polyinosinic-polycytidylic acid (poly I:C) matured DCs. Cytolytic activity was induced by both maturing agents. These data indicate that IFN-beta/IL-3 DCs represent a promising cell population for the immunotherapy of cancer.

Original publication

DOI

10.1111/j.1365-2249.2005.02700.x

Type

Journal article

Journal

Clin Exp Immunol

Publication Date

03/2005

Volume

139

Pages

468 - 475

Keywords

CD40 Ligand, CD8-Positive T-Lymphocytes, Cancer Vaccines, Cell Differentiation, Cell Line, Tumor, Dendritic Cells, Flow Cytometry, Humans, Interferon-beta, Interferon-gamma, Interleukin-3, Interleukin-5, Lymphocyte Activation, Lymphoma, T-Lymphocytes, Cytotoxic