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BACKGROUND AND OBJECTIVES: Mesenchymal stem/progenitor cells (MSCs) are multipotent progenitors that differentiate into such lineages as bone, fat, cartilage and stromal cells that support haemopoiesis. Bone marrow MSCs can also contribute to cardiac repair, although the mechanism for this is unclear. Here, we examine the potential of MSCs from different sources to generate cardiomyocytes in vitro, as a means for predicting their therapeutic potential after myocardial infarction. MATERIALS AND METHODS: Mesenchymal stem/progenitor cells were isolated from the perivascular tissue and Wharton's jelly of the umbilical cord and from cord blood. Their immunophenotype and differentiation potential to generate osteoblasts, chondrocytes, adipocytes and cardiomyoxcytes in vitro was compared with those of bone marrow MSCs. RESULTS: Mesenchymal stem/progenitor cells isolated from umbilical cord and cord blood were phenotypically similar to bone marrow MSCs, the exception being in the expression of CD106, which was absent on umbilical cord MSCs, and CD146 that was highly expressed in cord blood MSCs. They have variable abilities to give rise to osteoblasts, chondrocytes and adipocytes, with bone marrow MSCs being the most robust. While a small proportion (approximately 0.07%) of bone marrow MSCs could generate cardiomyocyte-like cells in vitro, those from umbilical cord and cord blood did not express cardiac markers either spontaneously or after treatment with 5-azacytidine. CONCLUSION: Although MSCs may be useful for such clinical applications as bone or cartilage repair, the results presented here indicate that such cells do not generate cardiomyocytes frequently enough for cardiac repair. Their efficacy in heart repair is likely to be due to paracrine mechanisms.

Original publication

DOI

10.1111/j.1423-0410.2008.01076.x

Type

Journal article

Journal

Vox Sang

Publication Date

08/2008

Volume

95

Pages

137 - 148

Keywords

Adipocytes, Adult, Antigens, Differentiation, Azacitidine, Bone Marrow Cells, Cell Differentiation, Cell Lineage, Cells, Cultured, Chondrocytes, Feasibility Studies, Fetal Blood, Gene Expression Profiling, Humans, Immunophenotyping, Infant, Newborn, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells, Muscle Proteins, Myocytes, Cardiac, Organ Specificity, Osteoblasts, Umbilical Cord