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The tumour suppressor gene causing multiple endocrine neoplasia type 1 (MEN1) encodes a 610 amino acid protein, menin. In order to identify menin-interacting proteins we used a yeast two-hybrid assay to screen a 12.5-dpc mouse embryo library with partial menin encompassing amino acids 278 to 476. This identified a homeobox containing protein encoded by a placenta and embryonic expression gene, referred to as Pem. GST-pull-down and coimmunoprecipitation experiments confirmed the interaction. Both proteins colocalised predominantly in the nucleus but were occasionally also found in the cytoplasm. Furthermore, in situ hybridisation studies revealed similarities in their expression patterns in mouse embryos and adult tissues. In adult mice both Men1 and Pem yielded strong signals in testis, Sertoli cells and particularly in seminiferous tubules. Thus, our study has identified that menin interacts with Pem, and the high expression of these proteins in the testis suggests a role in spermatogenesis.

Original publication

DOI

10.1006/bbrc.2001.5405

Type

Journal article

Journal

Biochem Biophys Res Commun

Publication Date

17/08/2001

Volume

286

Pages

426 - 431

Keywords

Animals, Cell Nucleus, Fluorescent Antibody Technique, Homeodomain Proteins, In Situ Hybridization, Male, Mice, Neoplasm Proteins, Proto-Oncogene Proteins, RNA, Messenger, Spermatogenesis, Testis, Transcription Factors, Two-Hybrid System Techniques