Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

CD2 and CD4 are single chain transmembrane T cell surface molecules that are involved in signal transduction. Chimaeric constructs from rat CD2 and CD4 antigens were expressed in the Jurkat human T cell line to examine the role of extracellular, transmembrane and cytoplasmic domains in mediating functions controlled by CD2 and CD4. The results show that the large rise in concentration of cytoplasmic free Ca2+ mediated via CD2 crosslinking is controlled by the cytoplasmic domain and does not require the CD2 transmembrane and extracellular domains. Similarly the CD4 cytoplasmic domain alone was shown to encode the specificity for binding to the p56lck tyrosine kinase and to control down-modulation of CD4 after treatment with phorbol ester. Evidence was obtained that down-modulation of CD4 occurs when p56lck dissociates from the cytoplasmic domain due to phosphorylation of Ser 405.

Type

Journal article

Journal

EMBO J

Publication Date

02/1991

Volume

10

Pages

377 - 385

Keywords

Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD2, Antigens, CD4, Antigens, Differentiation, T-Lymphocyte, Antigens, Surface, Base Sequence, Calcium, Cell Line, Cell Membrane, Chimera, Cytoplasm, Humans, Molecular Sequence Data, Oligonucleotide Probes, Rats, Receptors, Immunologic, Sequence Homology, Nucleic Acid, Signal Transduction, T-Lymphocytes, Tetradecanoylphorbol Acetate