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There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.

Original publication

DOI

10.1016/j.jim.2006.07.022

Type

Journal article

Journal

J Immunol Methods

Publication Date

20/10/2006

Volume

316

Pages

153 - 157

Keywords

Cell Adhesion, Cell Line, Flow Cytometry, Fluoresceins, Fluorescent Dyes, Humans, Keratinocytes, Rhodamines, Succinimides, T-Lymphocytes, Cytotoxic