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This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.

Original publication

DOI

10.1016/j.ymeth.2004.01.003

Type

Journal article

Journal

Methods

Publication Date

08/2004

Volume

33

Pages

302 - 311

Keywords

Animals, Cytoplasmic Granules, Exocytosis, Islets of Langerhans, Synaptic Vesicles