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BACKGROUND: While modulation of T cell function is believed to be important in the successful acquisition of clinical tolerance during venom immunotherapy, little is known of the role of wasp venom specific T cell antigens. OBJECTIVE: We sought comprehensively to characterize the T cell proteome for wasp venom to facilitate the future development of T cell-based immunotherapeutic approaches. METHODS: Using peripheral blood mononuclear cells from wasp venom-allergic individuals and IL-4 ELISPOT analysis, we characterized T cell responses to whole venom and gel filtration/ion exchange-fractionated venom. Reactive fractions were purified and identified using highly sensitive electrospray ion-trap mass spectrometry. RESULTS: Wasp venom-allergic individuals have detectable whole wasp venom-specific T cells directly ex vivo, which show rapid IL-4 effector function. T cell responses to gel filtration/ion exchange fractionated venom were dominated by responses to phospholipase A(1), hyaluronidase and antigen 5. CONCLUSION: Although it is likely that there are many T cell antigens within wasp venom, the main responses are to proteins coincident with the known IgE-binding proteins.

Original publication

DOI

10.1111/j.1365-2222.2006.02569.x

Type

Journal article

Journal

Clin Exp Allergy

Publication Date

10/2006

Volume

36

Pages

1274 - 1280

Keywords

Adult, Animals, Antigens, Case-Control Studies, Chromatography, Gel, Chromatography, Ion Exchange, Desensitization, Immunologic, Female, Humans, Hyaluronoglucosaminidase, Hypersensitivity, Immediate, Interleukin-4, Male, Middle Aged, Phospholipases, Proteome, Receptors, IgE, Spectrometry, Mass, Electrospray Ionization, T-Lymphocytes, Wasp Venoms