Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We generated five lines of transgenic mice carrying 1-3 copies of the Hb Lepore deltabeta fusion gene, in the context of a Bacterial Artificial Chromosome containing the whole human beta globin gene cluster. Normal developmental regulation of human genes occurred at levels approximating to those of endogenous genes. Deltabeta transgene expression became detectable during fetal life and rose to a mean level of 13.0% in adults, similar to that of humans. Low levels of human gamma chains were detectable as F cells in adult mice, but numbers did not increase after treatment with drugs that raise F cells in human subjects, even on a thalassaemic background.

Original publication

DOI

10.1111/j.1365-2141.2006.06354.x

Type

Journal article

Journal

Br J Haematol

Publication Date

12/2006

Volume

135

Pages

735 - 737

Keywords

Animals, Base Sequence, Chromosomes, Artificial, Bacterial, Gene Expression, Gene Expression Regulation, Developmental, Globins, Hemoglobins, Hemoglobins, Abnormal, Humans, Mice, Mice, Transgenic, Molecular Sequence Data, Peptide Fragments, Reticulocyte Count