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The actions of glucagon-like peptide-1(7-36)amide (GLP-1(7-36)amide) on cellular signalling were studied in human embryonal kidney 293 (HEK 293) cells stably transfected with the cloned human GLP-1 receptor. The cloned GLP-1 receptor showed a single high-affinity binding site (Kd = 0.76 nM). Binding of GLP-1(7-36)amide stimulated cAMP production in a dose-dependent manner (EC50 = 0.015 nM) and caused an increase in the intracellular free Ca2+ concentration ([Ca2+]i). The latter effect reflected Ca(2+)-induced Ca2+ release and was suppressed by ryanodine. We propose that the ability of GLP-1(7-36)amide to increase [Ca2+]i results from sensitization of the ryanodine receptors by a protein kinase A dependent mechanism.

Type

Journal article

Journal

FEBS Lett

Publication Date

09/10/1995

Volume

373

Pages

182 - 186

Keywords

Acetylcholine, Calcium, Calcium Channel Blockers, Cell Line, Cloning, Molecular, Cyclic AMP, Embryo, Mammalian, Glucagon, Glucagon-Like Peptide 1, Glucagon-Like Peptide-1 Receptor, Glucagon-Like Peptides, Humans, Ionomycin, Kidney, Peptide Fragments, Receptors, Glucagon, Recombinant Proteins, Ryanodine, Thionucleotides