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In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration [( Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent beta-cells.

Type

Journal article

Journal

FEBS Lett

Publication Date

21/10/1991

Volume

291

Pages

310 - 314

Keywords

Adult, Aged, Calcium, Cytoplasm, Female, Fura-2, Humans, Insulinoma, Islets of Langerhans, Male, Tumor Cells, Cultured