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The recombinant plasmids p324, p330 and p323 carrying the aminoglycoside phosphotransferase (aph) gene and 5' flanking c-myc sequences linked to the reporter gene chloramphenicol acetyl-transferase (cat) were introduced into the mouse erythroleukemia cell line F412B2TK- and stable transfectants resistant to geneticin were obtained. The effects of three anthracycline compounds, doxorubicin, daunorubicin and 4'-epidoxorubicin, were studied using a large range of drug concentrations. It was found that 4'-epidoxorubicin and daunorubicin stimulate cat-gene expression with maximum effect at a concentration of 1 micrograms/ml, while doxorubicin enhances transcription from c-myc promoter at 5 micrograms/ml.

Type

Journal article

Journal

Anticancer Res

Publication Date

11/1991

Volume

11

Pages

2153 - 2157

Keywords

Amino Acid Sequence, Animals, Daunorubicin, Doxorubicin, Epirubicin, Gene Expression Regulation, Neoplastic, Genes, myc, Leukemia, Erythroblastic, Acute, Mice, Molecular Sequence Data, Promoter Regions, Genetic