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The amounts of cytochromes and flavoproteins in insulin-producing RINm5F-cells were investigated with dual wavelength spectroscopy. The cytochromes a, a3, b and c were present in normal stoichiometric proportions. Whereas each cytochrome occurred in a concentration corresponding to 25-75 mumol/kg protein, the total amount of flavoproteins was equivalent to about 200 mumol/kg protein. In support of the existence of a functional respiratory chain, the cytochromes reacted normally both to additions of oxygen and to the inhibitors antimycin A and amytal.

Type

Journal article

Journal

Biomed Biochim Acta

Publication Date

1985

Volume

44

Pages

49 - 54

Keywords

Animals, Cytochromes, Energy Metabolism, Flavoproteins, Glycolysis, Islets of Langerhans, Mitochondria, Oxidation-Reduction