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A gene called deleted in colon cancer (DCC) has been identified on a region of chromosome 18, which is deleted in 70% of colorectal cancers. The DCC gene encodes a protein belonging to the immunoglobulin superfamily with similarity to the N-CAM transmembrane proteins and is a putative tumor-suppressor gene. Alternative splicing of transcripts of transmembrane proteins, including N-CAM, is known to occur, resulting in different isoforms of the protein. Using five antibodies against the DCC gene product (three monoclonal antibodies raised in our laboratory, one commercially available antibody, and a rabbit polyclonal antibody), we have demonstrated by immunostaining a DCC protein isoform in reticuloendothelial cells in human thymus, tonsil, and lymph node. This can be distinguished from another isoform described in normal colonic epithelium, because this latter is not demonstrable with the antibodies we have used. It could not be detected in normal colonic epithelium, polyps or colorectal carcinomas. This restrictive distribution suggests that not all DCC gene products are important in colonic cancer.

Type

Journal article

Journal

Cancer Res

Publication Date

01/12/1995

Volume

55

Pages

5628 - 5631

Keywords

3T3 Cells, Animals, Antibodies, Monoclonal, Cell Adhesion Molecules, Colon, Colorectal Neoplasms, DCC Receptor, Humans, Intestinal Polyps, Kidney Glomerulus, Lymphoid Tissue, Mice, Receptors, Cell Surface, Tumor Cells, Cultured, Tumor Suppressor Proteins