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Cellular decision-making is mediated by a complex interplay of external stimuli with the intracellular environment, in particular transcription factor regulatory networks. Here we have determined the expression of a network of 18 key haematopoietic transcription factors in 597 single primary blood stem and progenitor cells isolated from mouse bone marrow. We demonstrate that different stem/progenitor populations are characterized by distinctive transcription factor expression states, and through comprehensive bioinformatic analysis reveal positively and negatively correlated transcription factor pairings, including previously unrecognized relationships between Gata2, Gfi1 and Gfi1b. Validation using transcriptional and transgenic assays confirmed direct regulatory interactions consistent with a regulatory triad in immature blood stem cells, where Gata2 may function to modulate cross-inhibition between Gfi1 and Gfi1b. Single-cell expression profiling therefore identifies network states and allows reconstruction of network hierarchies involved in controlling stem cell fate choices, and provides a blueprint for studying both normal development and human disease.

Original publication

DOI

10.1038/ncb2709

Type

Journal article

Journal

Nat Cell Biol

Publication Date

04/2013

Volume

15

Pages

363 - 372

Keywords

Animals, Biomarkers, Tumor, Bone Marrow, Cell Differentiation, Cells, Cultured, Chromatin Immunoprecipitation, Gene Expression Profiling, Gene Regulatory Networks, Hematopoietic Stem Cells, Humans, Luciferases, Mice, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis, RNA, Messenger, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Single-Cell Analysis