Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

To investigate how early events in antigen processing affect the repertoire of peptides presented by MHC class I molecules, we compared the presentation of the influenza A nucleoprotein epitope 265-273 by HLA-A3 class I molecules in human and mouse cells. Mouse cells that express HLA-A3 failed to present the NP265-273 peptide when contained within the full-length nucleoprotein, to HLA-A3-restricted human cytotoxic T lymphocytes. However, when the epitope was generated directly in the cytosol using a recombinant vaccinia virus that expressed the nonamer peptide, mouse cells were recognized by HLA-A3-restricted CTL. Poor transport of the peptide by mouse TAP was not responsible for the defect as co-infection of mouse cells with recombinant vaccinia viruses encoding the full-length nucleoprotein and the human TAP1 and TAP2 peptide transporter complex failed to restore presentation. These results therefore demonstrate a differential processing of the influenza nucleoprotein in mouse and human cells. This polymorphism influences the repertoire of peptides presented by MHC class I molecules at the cell surface.

Original publication

DOI

10.1002/(SICI)1521-4141(199802)28:02<625::AID-IMMU625>3.0.CO;2-I

Type

Journal article

Journal

Eur J Immunol

Publication Date

02/1998

Volume

28

Pages

625 - 635

Keywords

ATP Binding Cassette Transporter, Subfamily B, Member 2, ATP Binding Cassette Transporter, Subfamily B, Member 3, ATP-Binding Cassette Transporters, Animals, Antigen Presentation, Antigens, Viral, Biological Transport, Endoplasmic Reticulum, Epitopes, HLA-A3 Antigen, Humans, Influenza A virus, L Cells (Cell Line), Mast-Cell Sarcoma, Mice, Nucleoproteins, RNA-Binding Proteins, T-Lymphocytes, Cytotoxic, Transfection, Tumor Cells, Cultured, Viral Core Proteins