Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The question of whether enhanced memory T cell responses are simply due to an increased frequency of specific cells or also to an improved response at the single cell level is widely debated. In this study, we analyzed T cell receptor (TCR) transgenic memory T cells and bona fide memory T cells isolated from virally infected normal mice using the tetramer technology. We found that memory T cells are qualitatively different from naive T cells due to a developmentally regulated rearrangement of the topology of the signaling machinery. In naive cytotoxic T cells, only a few CD8 molecules are associated with Lck and the kinase is homogeneously distributed inside the cell. However, in vivo priming of naive T cells induces the targeting of Lck to the CD8 coreceptor in the cell membrane and the consequent organization of a more efficient TCR signaling machinery in effector and memory cells.

Type

Journal article

Journal

J Exp Med

Publication Date

17/05/1999

Volume

189

Pages

1521 - 1530

Keywords

Acetyltransferases, Animals, Antigens, CD28, CD8-Positive T-Lymphocytes, Calcium, Cells, Cultured, Flow Cytometry, Immunologic Memory, Lymphocyte Activation, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Lymphocytic choriomeningitis virus, Mice, Mice, Transgenic, Microscopy, Fluorescence, Proteins, Receptors, Antigen, T-Cell, Signal Transduction, Spleen