Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples.
Kyes S., Craig AG., Marsh K., Newbold CI.
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.