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Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.

Original publication

DOI

10.1006/expr.1993.1108

Type

Journal article

Journal

Exp Parasitol

Publication Date

12/1993

Volume

77

Pages

473 - 483

Keywords

Animals, Antigens, Protozoan, Base Sequence, DNA, Protozoan, Molecular Sequence Data, Plasmodium falciparum, Polymerase Chain Reaction, Polymorphism, Genetic