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The use of interphase fluorescence in situ hybridisation (FISH) to study cytogenetic abnormalities in routinely fixed paraffin wax embedded tissue has become commonplace over the past decade. However, very few studies have applied FISH to routinely fixed bone marrow trephines (BMTs). This may be because of the acid based decalcification methods that are commonly used during the processing of BMTs, which may adversely affect the suitability of the sample for FISH analysis. For the first time, this report describes the simultaneous application of FISH and immunofluorescent staining (the FICTION technique) to formalin fixed, EDTA decalcified and paraffin wax embedded BMTs. This technique allows the direct correlation of genetic abnormalities to immunophenotype, and therefore will be particularly useful for the identification of genetic abnormalities in specific tumour cells present in BMTs. The application of this to routine clinical practice will assist diagnosis and the detection of minimal residual disease.

Original publication

DOI

10.1136/jcp.2005.026468

Type

Journal article

Journal

J Clin Pathol

Publication Date

12/2005

Volume

58

Pages

1336 - 1338

Keywords

Antigens, CD20, Antigens, Neoplasm, Biopsy, Bone Marrow Examination, Chromosome Aberrations, Edetic Acid, Formaldehyde, Hematologic Neoplasms, Humans, Immunophenotyping, In Situ Hybridization, Fluorescence, Paraffin Embedding