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The CITED family proteins bind to CBP/p300 transcriptional integrators through their conserved C-terminal acidic domain and function as coactivators. The 21-kDa mouse Cited4 protein, a novel member of the CITED family, interacted with CBP/p300 as well as isoforms of the TFAP2 transcription factor, coactivating TFAP2-dependent transcription. The cited4 gene consisted of only a single exon located on chromosome 4 at 56.5-56.8 cM flanked by marker genes kcnq4 and scml1. Expression of Cited4 protein was strong and selective in embryonic hematopoietic tissues and endothelial cells. In adult animals, Cited4 showed strong milk cycle-dependent induction in pregnant and lactating mammary epithelial cells. Strong induction of Cited4 expression was also observed in SCp2 mouse mammary epithelial cells during their prolactin-dependent in vitro differentiation. These results implied possible roles for Cited4 in regulation of gene expression during development and differentiation of blood cells, endothelial cells, and mammary epithelial cells.

Type

Journal article

Journal

Genomics

Publication Date

12/2002

Volume

80

Pages

601 - 613

Keywords

Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, COS Cells, Cloning, Molecular, DNA, DNA, Complementary, E1A-Associated p300 Protein, Embryo, Mammalian, Epithelial Cells, Exons, Female, Gene Expression, Gene Expression Regulation, Developmental, Genes, In Situ Hybridization, Introns, Male, Mammary Glands, Animal, Mice, Molecular Sequence Data, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Trans-Activators, Transcription Factors, Transcriptional Activation