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Polygalacturonase (Peh) and other pectolytic enzymes play a crucial role in the maceration of vegetables by soft rot Erwinia spp. We have sequenced the peh gene of Erwinia carotovora subsp. carotovora, and identified its product as a precursor of molecular weight 42,639, and a mature protein of molecular weight 42,200. A putative KdgR-binding site was identified in the region 5' to the peh gene. The Peh protein showed significant homology with Peh from tomato. In addition, we have found homologies between pectin methylesterase and pectate lyase from Erwinia and their counterparts in tomato. These homologies are described, and their significance discussed.

Original publication

DOI

10.1111/j.1365-2958.1990.tb00675.x

Type

Journal article

Journal

Mol Microbiol

Publication Date

06/1990

Volume

4

Pages

1029 - 1036

Keywords

Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial, Erwinia, Genes, Bacterial, Molecular Sequence Data, Polygalacturonase, Sequence Homology, Nucleic Acid