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The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.

Type

Journal article

Journal

AIDS

Publication Date

23/07/2004

Volume

18

Pages

1595 - 1598

Keywords

CD8-Positive T-Lymphocytes, Enzyme-Linked Immunosorbent Assay, Epitopes, Genes, gag, HIV Infections, HIV-1, Humans, Interferon-gamma