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We have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distribution. By defining positives as those that increase the concentration of folded heavy chains more than three standard deviations from the control we show that 7/48 bind A2.1, 11/49 bind Db and 7/47 bind Kb. The assembly assay contrasts with solid-phase assays in being more discriminating (fewer peptides binding any given class I molecule), and showing less overlap in the patterns of peptides bound by the three class I molecules.

Original publication

DOI

10.1002/eji.1830210909

Type

Journal article

Journal

Eur J Immunol

Publication Date

09/1991

Volume

21

Pages

2025 - 2031

Keywords

Amino Acid Sequence, Electrophoresis, Polyacrylamide Gel, Gene Products, gag, H-2 Antigens, HLA-A2 Antigen, Histocompatibility Antigens Class I, Humans, Immunoglobulin Heavy Chains, Lymphoma, Molecular Sequence Data, Precipitin Tests, Tumor Cells, Cultured, beta 2-Microglobulin