Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The transcription factor Runx1 marks all functional hematopoietic stem cells (HSCs) in the embryo and is required for their generation. Mutations in Runx1 are found in approximately 25% of acute leukemias and in familial platelet disorder, suggesting a role for Runx1 in adult hematopoiesis as well. A comprehensive analysis of Runx1 expression in adult hematopoiesis is lacking. Here we show that Runx1 is expressed in functional HSCs in the adult mouse, as well as in cells with spleen colony-forming unit (CFU) and culture CFU capacities. Additionally, we document Runx1 expression in all hematopoietic lineages at the single cell level. Runx1 is expressed in the majority of myeloid cells and in a smaller proportion of lymphoid cells. Runx1 expression substantially decreases during erythroid differentiation. We also document effects of reduced Runx1 levels on adult hematopoiesis.

Original publication

DOI

10.1634/stemcells.22-2-158

Type

Journal article

Journal

Stem Cells

Publication Date

2004

Volume

22

Pages

158 - 168

Keywords

Animals, Bone Marrow Cells, Cell Differentiation, Core Binding Factor Alpha 2 Subunit, DNA-Binding Proteins, Erythroid Precursor Cells, Female, Flow Cytometry, Hematopoietic Stem Cells, Lymphocytes, Male, Mice, Myeloid Cells, Proto-Oncogene Proteins, T-Lymphocytes, Transcription Factors