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The HMG-box transcription factor Sox2 plays a role throughout neurogenesis and also acts at other stages of development, as illustrated by the multiple organs affected in the anophthalmia syndrome caused by SOX2 mutations. Here we combined proteomic and genomic approaches to characterize gene regulation by Sox2 in neural stem cells. Chd7, a chromatin remodeling ATPase associated with CHARGE syndrome, was identified as a Sox2 transcriptional cofactor. Sox2 and Chd7 physically interact, have overlapping genome-wide binding sites and regulate a set of common target genes including Jag1, Gli3 and Mycn, genes mutated in Alagille, Pallister-Hall and Feingold syndromes, which show malformations also associated with SOX2 anophthalmia syndrome or CHARGE syndrome. Regulation of disease-associated genes by a Sox2-Chd7 complex provides a plausible explanation for several malformations associated with SOX2 anophthalmia syndrome or CHARGE syndrome. Indeed, we found that Chd7-haploinsufficient embryos showed severely reduced expression of Jag1 in the developing inner ear.

Original publication

DOI

10.1038/ng.825

Type

Journal article

Journal

Nat Genet

Publication Date

06/2011

Volume

43

Pages

607 - 611

Keywords

Animals, Anophthalmos, CHARGE Syndrome, Calcium-Binding Proteins, DNA-Binding Proteins, Ear, Inner, Gene Expression Regulation, Developmental, Hedgehog Proteins, Intercellular Signaling Peptides and Proteins, Jagged-1 Protein, Membrane Proteins, Mice, Mutation, Neural Stem Cells, Receptors, Notch, SOXB1 Transcription Factors, Serrate-Jagged Proteins