This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).
Journal article
2021-06-18T00:00:00+00:00
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Cell Biology, Cell isolation, Flow Cytometry/Mass Cytometry, Immunology, Metabolism, Adipose Tissue, Animals, Cell Culture Techniques, Cell Separation, Cells, Cultured, Female, Humans, Male, Mice, Stromal Vascular Fraction