Differentiation and Contractile Analysis of GFP-Sarcomere Reporter hiPSC-Cardiomyocytes.

Sharma A., Toepfer CN., Schmid M., Garfinkel AC., Seidman CE.

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) represent a powerful cellular platform for illuminating mechanisms of human cardiovascular disease and for pharmacological screening. Recent advances in CRISPR/Cas9-mediated genome editing technology underlie this profound utility. We have generated hiPSC-CMs harboring fluorescently-tagged sarcomeric proteins, which provide a tool to non-invasively study human sarcomere function and dysfunction. In this unit, we illustrate methods for conducting high-efficiency, small molecule-mediated differentiation of hiPSCs into cardiomyocytes, and for performing non-invasive contractile analysis through direct sarcomere tracking of GFP-sarcomere reporter hiPSC-CMs. We believe that this type of analysis can overcome sensitivity problems found in other forms of contractile assays involving hiPSC-CMs by directly measuring contractility at the fundamental contractile unit of the hiPSC-CM, the sarcomere. © 2018 by John Wiley & Sons, Inc.

DOI

10.1002/cphg.53

Type

Journal article

Journal

Curr Protoc Hum Genet

Publication Date

24/01/2018

Volume

96

Pages

21.12.1 - 21.12.12

Keywords

CRISPR, cardiomyocytes, contractility, drug screening, fluorescent reporter, iPSCs, pluripotent stem cells, sarcomere, Cell Differentiation, Green Fluorescent Proteins, Humans, Induced Pluripotent Stem Cells, Myocytes, Cardiac, Sarcomeres

Permalink Original publication