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Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation.

Original publication

DOI

10.1016/j.ymeth.2010.08.002

Type

Journal article

Journal

Methods

Publication Date

02/2011

Volume

53

Pages

151 - 162

Keywords

Animals, Binding Sites, Cell Differentiation, Chromatin Immunoprecipitation, Embryo, Mammalian, Embryo, Nonmammalian, Gene Expression Regulation, Mice, Systems Biology, Transcription Factors, Zebrafish