Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

BACKGROUND: Molecular testing is increasingly needed in colorectal carcinoma (CRC) and the current clinically relevant mutations are in BRAF, KRAS and NRAS. This study aimed to further validate a new alternative polymerase chain reaction (PCR) platform (Idylla, Biocartis) against existing next-generation sequencing (NGS) and immunohistochemistry (IHC) assays. METHODS: 56 Idylla tests were performed on 43 CRC cases, in a total of 74 comparisons against an NGS panel (Ion Torrent) and the VE1 (anti-BRAF) antibody IHC. Discrepant cases were also compared with either conventional (Cobas) or droplet digital PCR (Bio-Rad). RESULTS: Idylla showed an overall concordance of 100% (95% CI 93% to 100%) with comparator molecular testing and indications were that Idylla is likely to be more sensitive than routine NGS. BRAF IHC showed 90% concordance with NGS (95% CI 70% to 97%). CONCLUSIONS: This study validates Idylla in formalin-fixed, paraffin-embedded CRC tissue. BRAF IHC, however, is an unreliable substitute for molecular testing in CRC.

Original publication

DOI

10.1136/jclinpath-2017-204356

Type

Journal article

Journal

J Clin Pathol

Publication Date

07/2017

Volume

70

Pages

610 - 614

Keywords

COLORECTAL CANCER, IMMUNOHISTOCHEMISTRY, MOLECULAR PATHOLOGY, Colorectal Neoplasms, GTP Phosphohydrolases, Humans, Immunohistochemistry, Membrane Proteins, Mutation, Point-of-Care Systems, Proto-Oncogene Proteins B-raf, Proto-Oncogene Proteins p21(ras), Real-Time Polymerase Chain Reaction