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The interferon-induced protein kinase RNA activated (PKR) is activated after virus infection. This activation is transient during the human immunodeficiency virus type 1 (HIV-1) infection of lymphocytes, and the protein is not activated at the peak of infection. We observed that interferon-induced adenosine deaminase acting on RNA 1-p150 (ADAR1-p150) and ADAR1-p110 expression increases while the virus replicates actively. Furthermore, both forms of ADAR1 show enhanced interactions with PKR at the peak of HIV infection, suggesting a role for this protein in the regulation of PKR activation. We observed that ADAR1-p150, as previously shown for the TAR RNA binding protein (TRBP), reverses the PKR inhibition of HIV expression and production in HEK 293T cells. This activity requires the Z-DNA binding motif and the three double-stranded RNA binding domains but not the catalytic domain. In astrocytic cells, ADAR1-p150 increased HIV expression and production to an extent similar to that of TRBP. Small interfering RNAs against ADAR1-p150 moderately decreased HIV production. These results indicate that two interferon-induced proteins, ADAR1 and PKR, have antagonistic functions on HIV production. They suggest that ADAR1 and TRBP belong to a multiprotein complex that inhibits PKR during the HIV infection of lymphocytes.

Original publication

DOI

10.1128/JVI.02457-08

Type

Journal article

Journal

J Virol

Publication Date

10/2009

Volume

83

Pages

10119 - 10128

Keywords

Adenosine Deaminase, Amino Acid Motifs, Cell Line, Eukaryotic Initiation Factor-2, Gene Expression Regulation, Viral, HIV Infections, HIV-1, Humans, Jurkat Cells, Lymphocytes, Models, Biological, Phosphorylation, RNA-Binding Proteins, Transfection, Virus Replication, eIF-2 Kinase