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Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.

Original publication

DOI

10.1007/978-1-4939-3753-0_7

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2016

Volume

1448

Pages

85 - 93

Keywords

CD4+ T cells, CD45RA+, Human T cell, Lentiviral vector, Lentivirus, Primary, Titer