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The chromosome 14 inversion produces cytogenetic breakpoints at either end of the long arm of this chromosome. Previous studies have shown that a hybrid gene (designated IgT) consisting of an immunoglobulin VH gene segment and T cell receptor J alpha C alpha segments encompasses the telomeric breakpoint in SUP-T1, a cell line derived from a human T cell lymphoma. Here, we report that the centromeric breakpoint in SUP-T1 constitutes the reciprocal of a VH-J alpha join but involves gene segments different from those at the telomeric breakpoint. Therefore, chromosome inversion and IgT formation were mediated by two sequential VH-J alpha joining events. Moreover, sequences adjacent to the centromeric breakpoint detect a T-cell-specific RNA, encoded within the immunoglobulin VH locus, whose transcriptional activity may have facilitated the illegitimate VH-J alpha rearrangements.

Type

Journal article

Journal

Cell

Publication Date

03/07/1987

Volume

50

Pages

97 - 105

Keywords

Chromosome Inversion, Chromosome Mapping, Chromosomes, Human, Pair 14, DNA Restriction Enzymes, Humans, Immunoglobulin Heavy Chains, Immunoglobulin Variable Region, Lymphoma, Peptide Fragments, RNA, Messenger, RNA, Neoplasm, Receptors, Antigen, T-Cell, Receptors, Antigen, T-Cell, alpha-beta, Recombination, Genetic, T-Lymphocytes