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RNA-driven complementary DNA (cDNA) hybridization experiments have been carried out in order to detect complementary sequences in RNA prepared from a mouse T cell lymphoma line (EL4). In conditions where efficient hybridization of L-chain cDNA with homologous P3 myeloma mRNA was observed, poor hybridization was observed with EL4 mRNA unless a low criterion of hybrid formation was employed (i.e. hydroxyapatite fractionation). The hybrid formed between EL4 mRNA and L-chain cDNA was found to melt about 5 degrees C below the homologous hybrid indicating that the sequence detected in EL4 mRNA is similar but not identical with the P3 mRNA sequence. However, a similar sequence was detectable in a Clambda-producing myeloma cell line but in this line the concentration of the sequence was found to be an order of magnitude lower than in EL4 cells.

Original publication

DOI

10.1002/eji.1830070111

Type

Journal article

Journal

Eur J Immunol

Publication Date

01/1977

Volume

7

Pages

43 - 48

Keywords

Base Sequence, DNA, Hot Temperature, Immunoglobulin Light Chains, Immunoglobulin kappa-Chains, Lymphoma, Neoplasms, Experimental, Nucleic Acid Denaturation, Nucleic Acid Hybridization, Oligonucleotides, RNA, Messenger, RNA-Directed DNA Polymerase, T-Lymphocytes, Transcription, Genetic