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The expression and rearrangement of T cell rearranging (TRG) gamma genes in human leukemic cell lines has been examined. The cell line MOLT-17 produces abundant gamma mRNA which is translated into a protein found on the cell surface which is associated with the CD3 molecule. The analysis of the gamma mRNA sequences in MOLT-17, by cDNA cloning, shows transcripts of aberrantly rearranged genes as well as the productively rearranged allele. The productive allele consists of a rearranged V gamma 8 gene joined to J gamma 2. Two forms of aberrant transcript originate from the other rearranged gamma allele. One of these initiates just upstream of the unrearranged J gamma 2 segment, and the other initiates from a V gamma 8 gene segment joined to another J gamma segment, upstream of J gamma 2. An unusual feature of the latter transcript is that polyadenlyation has occurred at the end of the first exon of C gamma 2, where two conserved poly(A) addition signals occur. The MOLT-4 cell line, on the other hand, has productively and nonproductively rearranged gamma alleles, from which relatively little transcription occurs. These results define new J gamma segments in the human TRG gamma locus and suggest that positive activation of the gamma locus is necessary for high level transcription after rearrangement.

Original publication

DOI

10.1002/eji.1830171208

Type

Journal article

Journal

Eur J Immunol

Publication Date

12/1987

Volume

17

Pages

1729 - 1736

Keywords

Amino Acid Sequence, Antigens, Differentiation, T-Lymphocyte, Base Sequence, Cloning, Molecular, DNA, Disulfides, Gene Expression Regulation, Genes, Humans, Molecular Sequence Data, RNA, Messenger, Receptors, Antigen, T-Cell, Receptors, Antigen, T-Cell, gamma-delta, T-Lymphocytes, Transcription Factors, Transcription, Genetic, Tumor Cells, Cultured