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The LIM-only protein Lmo2, activated by chromosomal translocations in T-cell leukaemias, is normally expressed in haematopoiesis. It interacts with TAL1 and GATA-1 proteins, but the function of the interaction is unexplained. We now show that in erythroid cells Lmo2 forms a novel DNA-binding complex, with GATA-1, TAL1 and E2A, and the recently identified LIM-binding protein Ldb1/NLI. This oligomeric complex binds to a unique, bipartite DNA motif comprising an E-box, CAGGTG, followed approximately 9 bp downstream by a GATA site. In vivo assembly of the DNA-binding complex requires interaction of all five proteins and establishes a transcriptional transactivating complex. These data demonstrate one function for the LIM-binding protein Ldb1 and establish a function for the LIM-only protein Lmo2 as an obligatory component of an oligomeric, DNA-binding complex which may play a role in haematopoiesis.

Original publication

DOI

10.1093/emboj/16.11.3145

Type

Journal article

Journal

EMBO J

Publication Date

02/06/1997

Volume

16

Pages

3145 - 3157

Keywords

Adaptor Proteins, Signal Transducing, Animals, Basic Helix-Loop-Helix Transcription Factors, Binding Sites, COS Cells, Consensus Sequence, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, Genes, Reporter, Hematopoiesis, LIM Domain Proteins, Metalloproteins, Models, Genetic, Nuclear Proteins, Protein Binding, Proto-Oncogene Proteins, Regulatory Sequences, Nucleic Acid, TCF Transcription Factors, Transcription Factor 7-Like 1 Protein, Transcription Factors, Transcription, Genetic, Transcriptional Activation, Transfection