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The structure and rearrangement of the human T-cell receptor alpha-chain gene have been analysed. The constant region segment is comprised of four exons, with the 3' non-coding region present as a separate exon. Two J alpha segments have been identified by sequence analysis about 4 X 10(3) and 15 X 10(3) base-pairs upstream from the constant region segment. Analysis of alpha-chain rearrangements in a panel of T-cells reveals the presence of at least four more J alpha segments within 19 X 10(3) base-pairs of C alpha and suggests the existence of additional J alpha segments further upstream. This dispersed organization of the J alpha region contrasts with the clustered organization observed in other lymphoid rearranging genes. The use of J alpha probes in diagnostic purposes for T-cell leukaemia is not, therefore, convenient and will require a number of different probes to ensure complete analysis of the locus.

Type

Journal article

Journal

Mol Biol Med

Publication Date

06/1986

Volume

3

Pages

265 - 277

Keywords

Base Sequence, Cell Line, DNA, DNA Restriction Enzymes, Genes, Humans, Immunoglobulin Constant Regions, Immunoglobulin J-Chains, Leukemia, Receptors, Antigen, T-Cell, Recombination, Genetic, T-Lymphocytes