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DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.

Type

Journal article

Journal

Nature

Publication Date

18/03/1976

Volume

260

Pages

221 - 225

Keywords

DNA, Escherichia coli, Extrachromosomal Inheritance, Globins, Poly A, RNA, Messenger