Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

In addition to the major human immunoglobulin heavy-chain locus on chromosome 14q32.3, VH and D segments are known to be present on chromosomes 15 and 16. We have now amplified and sequenced 24 such VH segments from somatic cell hybrids and have assigned them to 15q11.2 and 16p11.2 using cosmid and yeast artificial chromosome clones. In addition, we have located a cluster of D segments on 15q11.2, previously thought to be located on 14q32.3. We propose that the segments on chromosome 16 arose by an interchromosomal duplication and identify the corresponding region on chromosome 14. Taken together with the completion of a map of the human VH locus on 14q32.3, the total number of VH segments now identified is 117. We can now account for most, if not all human germ-line VH segments.

Type

Journal article

Journal

Hum Mol Genet

Publication Date

06/1994

Volume

3

Pages

853 - 860

Keywords

Base Sequence, Chromosome Mapping, Chromosomes, Artificial, Yeast, Chromosomes, Human, Pair 14, Chromosomes, Human, Pair 15, Chromosomes, Human, Pair 16, Cosmids, DNA Primers, Genes, Immunoglobulin, Humans, Hybrid Cells, Immunoglobulin Fragments, Immunoglobulin Heavy Chains, Immunoglobulin Variable Region, In Situ Hybridization, Fluorescence, Karyotyping, Molecular Sequence Data, Multigene Family, Polymerase Chain Reaction