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We have identified and sequenced 14 human immunoglobulin VH segments cloned in a yeast artificial chromosome, and have used a rapid PCR-based technique (HAPPY mapping, 12) to derive the order and approximate distances between them. The sequences mapped comprise thirteen germline VH segments and one rearranged VH3 gene. Comparison of our map with other data suggests the existence of at least two distinct haplotypes, differing in the presence or absence of the consecutive genes DP-78, DP-46 and DP-64, and in the duplication of segments DP-49 and DP-65. Screening of ten individuals confirms the existence of both haplotypes, and indicates that both are common amongst the population.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

25/09/1993

Volume

21

Pages

4524 - 4529

Keywords

Base Sequence, Chromosome Mapping, Chromosomes, Artificial, Yeast, DNA Primers, Genes, Immunoglobulin, Haplotypes, Humans, Immunoglobulin Heavy Chains, Immunoglobulin Variable Region, Molecular Sequence Data, Multigene Family